Synthesis and Impurity Research of 2-Thioadenosine Monohydrate

2-Thioadenosine monohydrate (1) is a vital intermediate in the synthesis of cangrelor. However, its industrial-scale preparation process and analysis impurities formed during this remained largely unknown. Herein, cangrelor was synthesized from oxidate adenosine, key step involved compound 1 5. The effects parameters that influenced reaction, including reaction temperature time, were discussed. Moreover, four process-related purified, synthesized, identified via nuclear magnetic resonance spectroscopy high-resolution mass spectrometry. can be utilized to produce on kilogram scale, with high-performance liquid chromatography purity 98.0%. study sheds light helps drug manufacturers further understand formation